Philip II, King of Spain - bass
Don Carlo, Infante of Spain - tenor
Rodrigo, Marquis of Posa - baritone
The Grand Inquisitor - bass
Elisabeth of Valois - soprano
Princess Eboli - mezzo-soprano
A Monk - bass
Tybalt, page to Elisabeth - soprano
A Voice from Heaven - soprano
Count of Lerma - tenor
Royal Herald - tenor
Countess of Aremberg - mute
Flemish deputies, Inquisitors, Ladies and Gentlemen of the Spanish Court, the people, Pages, Guards, Monks, Soldiers - chorus